? TROUBLESHOOTING 68Multichannel-pipette 100 μl of diluted cells to each well of a 96-well plate. The remaining cell suspension can be kept and used for genotyping at the population level to gauge overall modification efficiency.69Inspect the colonies for a clonal appearance ~1 week after plating (rounded colonies radiating from a central point). Mark off the wells that may have been seeded with multiple cells.70Return the cells to the incubator and allow them to expand for 2–3 weeks. Refeed and replica-plate the cells as needed and as detailed in Steps 64 and 65.
