Chunk #66 — PROCEDURE — Isolation of clonal cell lines by dilution ● TIMING 2–3 h hands-on; 2–3 weeks expansion

Source

Genome engineering using the CRISPR-Cas9 system.

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▲ CRITICAL STEP Single-cell dissociation and accurate count of cell number are critical for clonal dilution. We recommend examining the dissociated cells under a microscope to ensure successful dissociation and recounting cells at an intermediate serial dilution stage to ensure accuracy.