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Chunk #2 — MATERIALS AND METHODS — Animals

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Lmo genes regulate behavioral responses to ethanol in Drosophila melanogaster and the mouse.
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C57BL/6J mice were used for single cell embryo injections and subsequent behavioral testing. CD1 females were used for embryo implantation. Animals were group housed. Food and water were provided at all times, and animals were on a 12-hour light-dark cycle. All animal protocols were approved by the Ernest Gallo Clinic and Research Center Institutional Animal Care and Use Committee.