Three different 19-nucleotide small interfering RNAs were designed to target mouse Lmo3 (NM_207222) using the Wistar siRNA design tool (Levenkova et al., 2004). The three 19-nucleotide targeting sequences are listed in the Supplemental Table S1. Target sequences, including the shScr control sequence, were incorporated into hairpins and cloned into the lentiviral vector pLL3.7 as described previously (Lasek et al., 2007). The shScr sequence encodes a nonspecific hairpin, predicted to not target any gene in the mouse genome. Lentivirus was produced from shScr and shLmo3.8 plasmids in 293FT cells using the ViraPower™ packaging mix (Invitrogen, Carlsbad, CA) as described previously (Lasek et al., 2007).
