Neuro-2a cells (American Type Culture Collection, Manassas, VA) were grown in DMEM plus 10% fetal bovine serum and 5% CO2. Cells were seeded into 12-well dishes, and transfected with pLL3.7 plasmids containing shLmo3 and shScr sequences using Lipofectamine™ 2000 and Opti-MEM media (Invitrogen) according to the manufacturer’s instructions. Forty-eight hours after transfection, RNA was isolated using the RNeasy® Mini Kit according to manufacturer’s instructions (Qiagen, Valencia, CA). Total RNA was treated with RNase-free DNase (Promega, Madison, WI) to remove genomic DNA contamination.
