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Chunk #5 — MATERIALS AND METHODS — Quantitative PCR

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Lmo genes regulate behavioral responses to ethanol in Drosophila melanogaster and the mouse.
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cDNA synthesis and TaqMan® quantitative PCR (qPCR) were performed as described in Lasek and colleagues (2007). The sequences of the mouse Lmo1, Lmo2, and Lmo3 primers are listed in the Supplemental Table S1. Predesigned primers to Lmo4 (Assay ID Mm00495373) and mouse Gapdh (product number 4352932E) were purchased from Applied Biosystems (Foster City, CA).