After the final mapping, unique splice junction data were collected and visualized using the UCSC genome browser (Figure 3 and 4A). Twenty splice junctions were found among the 22 splice junctions from the RefSeq Genes model. An additional 43 splice junctions were also discovered. Therefore, there are at least 65 splice junctions associated with GABAB1 (Figure 4). The 43 newly identified splice junctions are rare splicings. Other computational junction discovery approaches have identified rare splice junctions from other genes (20, 27). The TopHat false discovery rate is lower than 1% for rare splice junctions (20, 28).
