Chunk #27 — Methods — Mice

Source

Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells.

Embedded

yes

Text

For the in vivo point-mutation assay, mice carrying BigBlue λLIZ shuttle vector repeats (Stratagene) were crossed with Aldh2−/−Fancd2+/− mice on a C57BL/6 × 129S4S6/Sv hybrid background. The resulting mice were intercrossed to obtain Aldh2−/−Fancd2−/− BigBlue λLIZ and control mice.