For single HSC transplantation experiments, we used CD45.1 homozygous mice on a C57BL/6J × 129S6/Sv F1 background as recipients. CD45.1 (or Ptprca) had been serially backcrossed from B6.SJL onto 129S6/Sv for six generations, with selection at each generation by serotyping with anti-CD45.1 (A20, FITC, BioLegend) and anti-CD45.2 antibodies (104, PE-Cy7, BioLegend).
