Similarly to Aldh2−/−Fancd2−/− F1 mice, Aldh2−/−Fancd2−/−Trp53−/− mice were also generated in a C57BL/6 × 129S4S6/Sv F1 background. In brief, the Trp53 allele reported previously33 was backcrossed onto 129S6/Sv or C57BL/6J for six generations. Trp53+/− mice were then intercrossed with Aldh2−/−Fancd2+/− mice to establish parental (F0) strains on both genetic backgrounds, which were finally crossed to obtain Aldh2−/−Fancd2−/−Trp53−/− and control F1 mice.
