mice were crossed with Fanca+/−Ku70+/− Vav1-iCre to generate Fancafl/−Ku70−/−Vav1-iCre and control mice. The Vav1-iCre allele directs the expression of the iCre recombinase to HSCs and haematopoietic tissues32, and in this case yields the Fanca-null allele (FancaΔ or Fancatm1d(EUCOMM)Wtsi). FancaΔ/Δ mice phenocopy the Fanca−/− mice reported previously, as judged by FANCA expression, sterility and sensitivity to mitomycin C (Fig. 3, Extended Data Fig. 3).
