Pair-wise Fst values between samples were calculated using EIGENSOFT. Fst values indicate how much of the genetic variability between individuals from different populations is due to population affiliation. In our study, Fst was found to correlate considerably with geographic distances (r2 = 0.382, p-value≪0.01). Values ranged from ≤0.001 for neighbouring populations to 0.023 for Southern Italy and in a young subisolate of Finland (Kuusamo) (Table S2). The Fst distances between HapMap CEU sample and the other samples also correlated with geographic distance (r2 = 0.291, p-value<0.01). The German population sample showed zero Fst with the CEU sample whereas the Finns from Kuusamo and the southern Italians were most different from them (Fst = 0.013 and 0.008, respectively) (Table S2). Pair-wise Fst values for CEU and either Latvians, Lithuanians, Estonians or western Russians were intermediate (0.006, 0.005, 0.004 and 0.004, respectively).
