Two or more samples were available from several countries which allowed us to measure the intra-population variability by Fst. Mean Fst was 0.001 for the 14 Estonian counties, 0.005 for Finland, 0.000 for Germany and 0.005 Italy (each with two samples), and 0.007 for the HapMap CHB and JPT samples. Multi-sample populations were taken from the final PC map to demonstrate the substructure of the populations (Figure S3).
