Under isoflurane anesthesia, 26-G bilateral guide cannulae (Plastics One, Roanoke, VA, USA) were targeted to BLA (−1.40 mm anterior–posterior, ±3.30 mm mediolateral, −3.90 mm ventral to Bregma) and held in place with dental cement. Mice were singly housed and given a 7-day post-surgery recovery period during which dummy cannulae were replaced daily to habituate mice to handling and prevent cannulae blocking. On completion of testing, mice were perfused with 4% paraformaldehyde. Fixed brains were sectioned (50-μm thickness) on a vibrating microtome and stained with cresyl violet to verify the localization of the cannulae placements with the aid of a microscope.
