Chunk #23 — Materials and Methods — Effects of intra-amygdala infusions — Systemic AM3506 treatment + amygdala infusion of SR141716

Source

Convergent translational evidence of a role for anandamide in amygdala-mediated fear extinction, threat processing and stress-reactivity.

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AM3506 was administered at a dose of 0 or 1.0 mg/kg 60 min before extinction training. SR141716 (0 or 2 μg/μl) was bilaterally infused into the BLA 5 min before systemic treatment with 1.0 mg/kg AM3506. A control group of mice treated with vehicle instead of each drug was also included and tested in the same manner. The purpose of this experiment was to test whether the extinction-facilitating effect of AM3506 was dependent on the action at CB1R in the BLA. The effect of drug treatment on freezing during retrieval was statistically analyzed using ANOVA, followed by Newman–Keuls post hoc tests.