We ran LOKI, a linkage analysis package for quantitative traits within large and complex pedigrees which uses Markov chain Monte Carlo techniques to calculate kinship coefficients between any two individuals (www.helix.nih.gov/apps/bioinfo/loki.html). The numbers of relative pairs calculated using the kinship coefficient were as follows: 1st degree: 384; 2nd degree: 349; 3rd degree: 495; 4th degree: 547; <5% related: 1466. The percentage of genetic identity shared between any two individuals in the pedigrees through common descent was calculated for all possible pairs (related and unrelated) using S.A.G.E. (Case Western Reserve University). The average sharing of descent was 0.3%, which is less than the degree of relationship between third cousins.
