Chunk #30 — Materials and methods — Antibodies and reagents

Source

Alcohol exposure alters pre-mRNA splicing of antiapoptotic Mcl-1L isoform and induces apoptosis in neural progenitors and immature neurons.

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Antibodies were obtained from the following sources; mouse anti-Nestin antibody (BD Transduction Laboratories), mouse anti-Synaptophysin (7H12, Cell Signaling Technology), rabbit anti-Map2 (D5G1, Cell Signaling Technology), anti-Cleaved Caspase-3 (Ab-2, Millipore-Sigma Aldrich), anti-PUMAα/β Antibody (G-3, Santa Cruz Biotechnology, Inc.), anti-Bcl-2 (C-2, Santa Cruz Biotechnology, Inc.), anti-Mcl-1 (S-19, Santa Cruz Biotechnology, Inc.), anti-Bax (N-20, Santa Cruz Biotechnology, Inc.), anti-Bad (Cell Signaling Technology), anti-SRSF1 (3G268, Santa Cruz Biotechnology, Inc.), anti-Tubulin (Millipore-Sigma-Aldrich). Mammalian protease inhibitors were obtained from Sigma-Aldrich (St Louis, MO). Bradford reagent was from BioWorld (Dublin, OH). MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) was obtained from Thermo Fisher Scientific (#M6494).