An additional limitation of this method is the requirement of a large starting sample is needed since dividing the sample into genetically homogenous subpopulations leads to smaller groups. Indeed, significant structure remained in the SAS (n = 584) and AMR super-populations (n = 659) but further dividing the populations would yield problematically small sample sizes. Therefore, the need to create more homogenous subpopulations must be balanced against losing power from small subsamples.
