Comparisons were made of animals tested in identical conditions. Relative speeds (treated/untreated × 100) were used in comparisons. We used Prism 5.0 software (GraphPad, La Jolla, CA) to perform 2-way ANOVA comparisons across time of ethanol exposure and genotype with Bonferroni post-hoc comparisons at each time point to determine differences between genotypes. Development of AFT was tested using a paired two-tailed t-test.