Cells were harvested with RNABee (Amsbio, CS-105B) at room temperature for 5 min. Lysates were transferred to a 1.5 ml micro-tube. Chloroform was mixed with RNABee at one fifth of the total volume. Samples were incubated on ice for 5 min then centrifuged for 15 min at 4 °C at 12,000g. The aqueous phase was collected and an equivalent volume of isopropanol was added, mixed and centrifuged again for 15 min at 4 °C at 12,000g. The RNA pellet was washed two times with RNAse-free 75% ethanol and dissolved in DEPC-treated water.
