A spine was considered immunolabeled if a PEG particle occurred anywhere within the spine. One-way ANOVA indicated no effect of the treatment upon the proportion of spines labeled generally (Fig. 6A). The proportions of spines immunolabeled at nonsynaptic sites were also not significantly different across the treatment groups (Fig. 6B). However, rats in the paired group showed a 99% increase in the proportion of spines immunolabeled at the synaptic junction (Zones I–III), compared with the spines of naïve rats, and this effect was statistically significant (F(2,8) = 5.5694, P < 0.05) (Fig. 6C). Spines of the LA from the unpaired group were also elevated at the synaptic junction, relative to the spines of naïve rats, but this change did not reach statistical significance (P = 0.10) (Fig. 6C).
