Briefly, glioma cells were transfected with WT LINC00665 or Mut LINC00665 plasmid with the putative binding site mutation. Cell lysates were obtained in which magnetic bead-conjugated TAF15 and STAU1 antibodies (IgG as a control) were added, respectively. Protein complexes were obtained after incubation, centrifugation, and rinsing. Proteinase K digestion and extraction of RNA was performed with TRIzol reagent. The expression level of LINC00665 was detected by quantitative real-time PCR.
