Rats were placed in a vapor chamber with normal air for one week for habituation to a new environment (two rats per cage). Then alcohol vapor exposure was slowly increased in the course of 1 week until blood alcohol concentration (BAC) reached ~200 mg/dL. Rats were exposed to alcohol vapor for 7 weeks (5 days a week, 14 h/day) or left unexposed. BAC was measured once per week from one rat per cage (rats were alternating every other week). One week after the last alcohol exposure, animals were sacrificed, and brain samples were collected.
