We obtained WES data from unaffected parent-child trios (n = 625) from the Simons Simplex Collection (SSC) (De Rubeis et al., 2014; Dong et al., 2014; Iossifov et al., 2012, 2014; Neale et al., 2012; O’Roak et al., 2011, 2012; Sanders et al., 2012, 2015; Willsey et al., 2013). These children and their parents have no evidence of autism spectrum or other neurodevelopmental disorders (Fischbach and Lord, 2010b). Like the TIC Genetics data, these SSC data were generated from primary blood-derived DNA and sequenced on the Illumina HiSeq 2000 sequencing platform after capture with the NimbleGen SeqCap EZ Exome v2 array.
