We performed whole-exome capture and sequencing of DNA from 511 affected children and their parents (1,533 samples total). We derived all DNA samples from primary blood cells. Of the 511 trios, we sequenced 325 (TIC Genetics) at the Yale Center for Genomic Analysis (YCGA), using the NimbleGen SeqCap EZ Exome v2 capture library (Roche NimbleGen, Madison, WI, USA) and the Illumina HiSeq 2000 sequencing platform (74 bp paired-end reads; Illumina, San Diego, CA); 149 (TSAICG) at the Broad Institute, using the Agilent SureSelect v1.1 capture library (Agilent Technologies, Santa Clara, CA, USA) and Illumina HiSeq 2500 sequencing platform; and 37 (TSAICG) at UCLA using the NimbleGen SeqCap EZ Exome v3 capture library (Roche NimbleGen, Madison, WI, USA) and the Illumina HiSeq 2500 sequencing platform.
