Finally, we analyzed the LPHN3-OLF mutation Ala313Ser reported in an ADHD patient (Domene et al., 2011). The mutant protein is expressed at levels similar to the WT protein and both hydrodynamic parameters and association with FLRT3-LRR were similar to the WT protein (Table 1). These data indicate that, Ala313, which is situated at a surface region distinct from the complex interface (~12 Å from the closest FLRT3-LRR residue), does not play an important role in the association with FRLT3-LRR.
