Chunk #9 — 2. Materials — 2.2. Equipment and software

Source

Differentiation of human pluripotent stem cells into Medial Ganglionic Eminence vs. Caudal Ganglionic Eminence cells.

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yes

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Laminar flow hoodCO2 incubator (Sanyo, Osaka, Japan)Refrigerator, 4 °C (Sanyo, Osaka, Japan)Inverted microscope with phase contrast (Olympus, Center Valley, PA, CK40)Centrifuge with swing-bucket rotor (Eppendorf, Hauppauge, NY, 5810)Pipet-aid (Drummond Scientific, Broomall, PA, cat. no. 4-000–101)Serological pipettes (25, 10 and 5 ml; BD Biosciences, San Jose, CA, cat. nos. 357535, 357551 and 357543, respectively)Pipetman (P1000, P200, P20 and P10; Gilson, Middleton, WI)Sterile pipette tips (1 ml, 200 and 10 µl; Fisher Scientific, Pittsburgh, PA, cat. nos. 02–681-165, 02–681-457 and 02–681-440, respectively)Sterile filter units, 500 ml (Thermo Scientific, Waltham, MA, cat. no. 566-0020)Tissue culture dishes, 100 mm (Fisher Scientific, Pittsburgh, PA, cat. no. 08–772-E)Conical tubes (50 and 15 ml; Fisher Scientific, Pittsburgh, PA, cat. nos. 14–959-49A and 14–959-49B, respectively)Ultra low attachment 6-well tissue culture plates (Corning, Corning, NY, cat. no. 3471)Cell lifters (Fisher Scientific, Pittsburgh, PA, cat. no. 08–100–240)Cover slips, 12 mm (Chemglass life Sciences, Vineland, NJ, cat. no. CLS-1760-012)Standard 24-well tissue culture plates (Corning, Corning, NY, cat. no. 353047)FACSAria III (BD Biosciences, San Jose, CA)FlowJo software (Tree Star, Ashland, OR, http://www.flowjo.com)Low-profile PCR tube strips, 0.2 ml (Bio-Rad Laboratories, Inc., Hercules, CA,