Chunk #10 — 2. Materials — 2.2. Equipment and software

Source

Differentiation of human pluripotent stem cells into Medial Ganglionic Eminence vs. Caudal Ganglionic Eminence cells.

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(Chemglass life Sciences, Vineland, NJ, cat. no. CLS-1760-012)Standard 24-well tissue culture plates (Corning, Corning, NY, cat. no. 353047)FACSAria III (BD Biosciences, San Jose, CA)FlowJo software (Tree Star, Ashland, OR, http://www.flowjo.com)Low-profile PCR tube strips, 0.2 ml (Bio-Rad Laboratories, Inc., Hercules, CA, cat. no. TLS-0801)Cap strips for PCR tubes (Bio-Rad Laboratories, Inc., Hercules, CA, cat. no. TCS-0803)DNA Engine Opticon (MJ Research, Waltham, MA)MacVector software (MacVector, Cary, NC, http://macvector.com)Inverted microscope with DSU spinning disk confocal (Olympus, Center Valley, PA IX81)Stereo Investigator image capture equipment and software (MBF Bioscience, Williston, VT, http://www.mbfbioscience.com/stereo-investigator.)Prism 6 software (GraphPad Software, La Jolla, CA, http://www.graphpad.com/scientific-software/prism)Angle Two stereotaxic for mouse (Leica Biosystems, Buffalo Grove, IL, cat. no. 39464610)Cunningham mouse stereotaxic adaptor (Harvard Apparatus, Holliston, MA, cat. no. 724794)Mobile isoflurane vaporstick system with small induction box (Harvard Apparatus, Holliston, MA, cat. no. 726422)Electric heating pad (Fisher Scientific, Pittsburgh, PA, cat. no. NC0331246)Cryostat (Leica Biosystems, Buffalo Grove, IL, CM1950)MetaMorph software (Molecular Devices, Sunnyvale, CA, http://www.moleculardevices.com/)