The experimental procedure involved one, 120-min session of habituation to the locomotor chambers, followed by alternate baseline sessions and test sessions. Animals received microinjections 1 min before every session. Habituation and baseline sessions involved microinjections of aCSF whereas test sessions involved one of the three treatments: aCSF, OxoSQ 1 nmol and OxoSQ 10 nmol per side. Thus a total of 7 microinjections (1 per day) were performed in these animals. The order of the three treatments given to each individual animal was randomized. Two additional sessions involved microinjections in the CIC with aCSF and OxoSQ 10 nmol per side.
