Eight animals from the food group were used to perform the locomotor activity tests. Microinjections were performed the same way as in food and cocaine groups before the animals were put in the locomotor chambers. The same treatments were delivered before the locomotor activity sessions. Locomotor activity sessions were conducted 2 hours into the dark phase and lasted for 120 min. Five Accuscan automated activity monitors (Accuscan Instruments Inc., Columbus, OH, USA) were used for measuring locomotor activity. The activity monitoring apparatus consisted of three major units: (1) a clear acrylic plastic test cage 40×40×30 cm set inside (2) a monitoring unit equipped with photocell beams and detectors that allowed measurement of total distance in centimeters (cm) via beam breaks; and (3) a black acrylic plastic chamber lined with foam to attenuate external environmental sounds that housed units (1) and (2). Total distance was measured in 5 min time periods.
