Comparative deuterium exchange mass spectrometry studies were carried out with LPHN3- OLF alone and with LPHN3-OLF/FLRT3 complex by mixing these proteins at 1:1 molar ratio. The exchange reactions were quenched at various time points by addition of 18μl of ice-cold 0.8% formic acid, 0.8M GuHCl, 16.6% glycerol for a final pH of 2.5. Peptide identification was done by the aid of Proteome Discoverer software (ThermoFisher).
