subsequent SAD phasing with PHENIX module PHASER, using four diffraction datasets collected with a 6 keV X-Ray beam. The subsequent 2.6 Å resolution initial model was further refined using Phenix.refine against separated collected P65 and C2221 data at 1.6 Å resolution, after a molecular replacement with the PHENIX module PHASER for the latter. The complex structure was solved by molecular replacement using the PHENIX module PHASER using the P65 structure of LPHN3-OLF and the recently determined structure of FLRT3-LRR (PDB ID 4V2E) as starting models. Subsequent refinement of the structure was performed using BUSTER.
