Crystals of LPHN3-OLF were made by under-oil microbatch method by mixing 1 μL of protein with an equal volume of crystallization solution containing [15–25]% polyethylene glycol 8000, [50–300] mM MgCl2 and 0.1 M TAPS pH 9.0. C2221 crystal forms were harvested from 50 mM MgCl2 containing conditions whereas P65 crystal forms grew in 300 mM MgCl2 containing conditions. Crystals of the complex were prepared by vapor diffusion in hanging drops by mixing 1 μL of a 1:1.2 molar ratio mixture of LPHN3-OLF and FLRT3-LRR with an equal volume of crystallization solution containing 6 % polyethylene glycol 3350 and 0.2 M NaNO3. Complete data sets were collected from individual crystals under a cryogenic stream at 100K. All diffraction data sets were processed by XDS and SCALA. For the LPHN3-OLF structure Native-SAD phasing was performed first by substructure solution with SHELXD and subsequent SAD phasing with PHENIX module PHASER, using four diffraction datasets collected with a 6 keV X-Ray beam. The subsequent 2.6 Å resolution initial model was further refined using Phenix.refine against separated collected P65 and C2221 data at 1.6 Å
