In sum, genome-wide significance threshold was set at P< 5x10-8 in the UHS cohort. Given prior successful identification of replicable SNP—disease associations from among signals that were not genome-wide significant in discovery, lower thresholds were used to select regions for follow-up in the WIHS (imputed variants with P<1x10-6 and the top genotyped SNP association with P = 1x10-5). Within the follow-up regions, variants that had a discovery P<1x10-3 within 3MB of the top variant were selected, a total of 692. Taking into account linkage disequilibrium among the 692 follow-up SNPs this constituted 156 independent tests for replication (P<3.21x10-4).
