Chunk #6 — Materials and Methods — Subjects, genotyping and assignment of nominal significance of dependent vs control allele frequencies in each sample — 1) dbGAP samples from the FSCD, COGA and COGEND studies
Genotypes from unrelated subjects who provided written consents and met DSM criteria for alcohol dependence and consenting control subjects with no evidence for dependence on any drug were assembled from three sets of subjects and deposited in dbGAP (http://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs000092.v1.p1). Family study of cocaine dependence (FSCD) subjects were recruited from treatment centers close to St. Louis. Mo; 55% of contacted subjects participated [22]. Community-based comparison subjects were recruited through driver's license records from the Missouri Family Registry and were matched to alcohol dependent subjects based on date of birth, ethnicity, gender, and zip code. Eighty percent of screened and eligible comparison subjects participated. Other participants came from individuals who participated in the Collaborative Study on the Genetics of Alcoholism (COGA) [23] and the Collaborative Study on the Genetics of Nicotine Dependence [10]. Dependent individuals displayed DSM (Diagnostic and Statistical Manual IV) dependence on alcohol. Controls, defined in dbGap variable phv00022939.v1.p1.c2 “final_type”, displayed no DSM dependence on alcohol, cocaine, marijuana, opioids or other drugs but may have evinced DSM nicotine dependence, FTND scores >4 and/or regular smoking as defined by smoking >100
