The female cyp2a5−/− and cyp2a5+/+ mice were fed HFD or CD for 3 months. After HFD feeding, the cyp2a5−/− mice exhibited a significant increase in body weight starting from the 6th week, while the significant increase in body weight was observed in cyp2a5+/+ mice starting from the 9th week. The body weight was increased in HFD-fed cyp2a5−/− mice to a greater extent than in HFD-fed cyp2a5+/+ mice starting from the 6th week (Fig 1 A). After HFD feeding, serum triglyceride was increased by 2-fold in cyp2a5−/− mice, but it was increased only 20% in cyp2a5+/+ mice (Fig 1 B). Similarly, serum glucose was not significantly increased in cyp2a5+/+ mice but it was increased about 2.5-fold in cyp2a5−/− mice (Fig 1 C). In response to glucose challenge, the HFD-fed cyp2a5−/− mice exhibited a more severe glucose intolerance than the HFD-fed cyp2a5+/+ mice did (Fig 1 D). However, after 30min of insulin injection, there was no significant difference in blood glucose reduction between the cyp2a5−/− mice and cyp2a5+/+ mice, either HFD-fed or CD-fed (Fig 1 E). After 2 h of insulin injection,
