Chunk #34 — Experimental Procedures — Acetylated LDL Uptake Assay and Tube Formation Assay

Source

In Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling.

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LDL uptake assays for ECs were performed as previously reported (Yamamizu et al., 2013). LDL uptake by cells was assessed by fluorescence microscopy after incubation with 10 μg/mL acetylated LDL labeled with 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindo-carbocyanine perchlorate (DiI-Ac-LDL) (Biomedical Technologies) for 4 hr at 37°C.