Chunk #35 — Experimental Procedures — Acetylated LDL Uptake Assay and Tube Formation Assay

Source

In Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling.

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The tube formation assay for ECs was carried out as described previously (Yamamizu et al., 2013). ECs and pericytes (total 7 × 104) at 12 days after differentiation were cultured in a 24-well plate (Life Technologies) coated with 150 μL of Matrigel Basement Membrane Matrix GFR (BD Biosciences).