RNA samples from thirty human livers were sequenced using SOLiD® technology (Thermo Fisher Scientific, Waltham, MA, United States). SNPs in the 3′ UTRs were identified (O’Leary et al., 2016) and an 8-base pair region on either side of the reference and variant alleles was analyzed using TargetScan (Lewis et al., 2005) to identify SNPs that were in miRNA seed binding regions. SNPs that altered the predicted miRNA seed binding sites were considered for further analysis. For assay development, 84 SNPs that were associated with allele-specific expression in the sequencing dataset were selected. A flowchart representing the selection process of the 84 test mirSNPs is shown in Supplementary Figure 1. In addition, we selected 16 mirSNPs from the SomamiR database (Bhattacharya et al., 2013) that have been linked with cancer. The list of 100 SNPs used for assay development are listed in Supplementary Table 1. Similarly, 111 mirSNPs located in the 3′ UTR regions of 17 pharmacogenes- the core absorption, distribution, metabolism, and excretion (ADME) genes1, PXR, CAR, and HNF4α which showed allele specific expression in the sequencing dataset were selected
