the pIIIu was dramatically reduced by D-Lys3-GHRP-6 as demonstrated by significant interaction between the effects of two injections [F(1,15) = 13.59, p = 0.002; Fisher’s PLSD post-hoc p < 0.0001 for the saline pre-treated ethanol-injected animals versus all other groups] (Fig. 4). This effect was specific for the pIIIu. For instance, D-Lys3-GHRP-6 did not have any effect on the expression of c-Fos seen after ethanol in the VTA (Fig. 4). In the Arc, there was no significant interaction between the two injections administered but there was a significant increase in the number of c-Fos cells in D-Lys3-GHRP-6-injected animals compared to saline as demonstrated by a significant effect of first injection [F(1,10) = 6.21, p = 0.032, ANOVA; Fisher’s PLSD post-hoc p =0.045 for all D-Lys3-GHRP-6-injected animals versus all saline-injected animals] (Fig. 4).
