The decrease in number of c-Fos immunopositive cells in the pIIIu after ethanol drinking or injection could also theoretically have been due to changes in BECs caused by D-Lys3-GHRP-6. To address this possibility, we determined BECs at two time-points, 1 h and 2 h, after a systemic injection of 2.5 g/kg ethanol in mice that were pre-treated with either 400 nmol D-Lys3-GHRP-6 or with saline. As can be seen in Fig.5, BECs after D-Lys3-GHRP-6 (274.0 ± 15.71 at 1 h and 203.94 ± 7.85 at 2 h) were not different when compared to saline (261.54 ± 6.38 at 1h and 192.77 ± 6.52 at 2 h) at either time-point.
