Neuronal cultures derived from hiPSC lines were treated with a small-molecule structure corrector, PH002 (final concentration, 100 nM; prepared in dimethyl sulfoxide) as described36–38. After 7 days of treatment, culture medium was collected for measurement of Aβ40 and Aβ42, and cells were homogenized for western blot analyses. For the dose-effect experiments, different doses of PH002 (i.e., 0 nM, 10 nM, 30 nM, and 100 nM) were used to treat the culture neurons for 1 week.
