Neuronal cultures derived from apoE−/− hiPSCs were treated with purified recombinant human apoE3 or apoE4 (final concentration, 220 nM or 7.5 µg/ml). After 2 days of treatment with recombinant human apoE, culture medium was collected for measurement of Aβ40 and Aβ42, and cells were homogenized for protein normalization and western blot analysis.
