Power analysis of a pilot cohort (n = 3) showed that a sample size of three would be sufficient to show genotype effects; all groups have n ≥3. Values are expressed as mean ± SEM. All n numbers represent the numbers of biological replicates or fields used for image analysis. The distribution of data was assessed with the Shapiro-Wilk normality test; most of the data were normally distributed. The variances between groups were similar as shown by Bartlett’s test. Statistical significance was calculated with GraphPad Prism (GraphPad Software). Differences between groups were determined with the unpaired two-sided t test. For multiple comparisons, one-way analysis of variance (ANOVA) with Tukey’s post-test was used. P < 0.05 was considered significant. Researchers were not blinded to genotypes during experiments.
