We analyzed the effect of deleting Zfhx1b using DlxI12b-Cre at E12.5 and E15.5. In general, all of the phenotypes of MGE-derived cells observed with the Nkx2.1-Cre were recapitulated with the DlxI12b-Cre (Figures 3, S1, S3), including the strong reduction of tangential migration to the cortex, indicated by analysis of Cre-dependent reporter EGFP expression, and Lhx6, Sst, and CXCR7 expression. Like Nkx2.1-Cre mutants, DlxI1/2b-Cre mutants showed increased numbers of striatal cells that expressed Lhx6, Nkx2-1, NPY, Sst and Sox6 (Figures 3A-3I’, 3M-3O’ and S3J-S3L’). Furthermore, these mutants did not show an increase in the number of cells that expressed markers of the globus pallius (Lhx8, Gbx2, Kcnmb4) or striatal cholinergic interneurons (Lhx8, Gbx2) (Figures 3J-3L’, 3P-3R’; and S1Q’-S1S’, and data not shown).
