We assessed the ability of the top signals (p-val < 1.0×10−5) from the meta-analysis to replicate in a separate cohort of 45,773 cases and 106,354 controls from 23andMe (Table 1). All individuals in the replication dataset were independent from subjects included in the discovery 23andMe dataset and had similar characteristics for sex and age distributions. The replication cohort provided additional support for three of the five genome-wide significant SNPs in the TMEM161B-MEF2C (2 SNPs) and the NEGR1 locus. In a joint-analysis of the discovery 23andMe dataset, PGC, and the 23andMe replication dataset, a total of 15 independent loci (17 SNPs) reached genome-wide significance (p-val < 5×10−8) (Table 2). Of the remaining 46 SNPs with a p-value less than 1×10−5 in the meta-analysis of the 23andMe Discovery dataset and 23andMe, 41 had a consistent direction of effect between the meta-analysis and replication cohort (pvalues across all analyses including joint-analysis are shown in Supplementary Table 2 for SNPs that reached a pval < than 1×10−5 in the meta-analysis).
