Adult male mice were treated with cocaine (20 mg/kg) for 7 days and nucleus accumbens was then harvested for preparation of RNA (Figure 8). Utilization of full-length Kalrn promoters A, B, C and D was first evaluated; following injection of saline or cocaine, Kalrn promoter C accounted for most of the full-length transcripts produced (Figure 8A). Usage of the ΔKalrn promoter was next compared to the total level of full-length Kalrn transcript (Figure 8B). Chronic cocaine treatment increased usage of the ΔKalrn promoter without altering expression of full-length transcripts (Figure 8B).
