The PASSPORT-seq assay was conducted as previously described [13] with some modifications described in Supplementary Materials. Briefly, the procedure includes the following steps: oligonucleotide synthesis, plasmid library construction, transfection, DNA/RNA extraction, and sequencing. The oligonucleotide pool was synthesized commercially (Oligomix®, LC Sciences, Houston, TX, USA), and included 874 DNA oligos to target both alleles of 437 SNPs in the 3′UTR of genes that showed differences in allelic ratio between control and AUD groups. These were cloned in parallel into the 3′UTR of the luciferase gene in the pIS-0 vector (12178, Addgene, Cambridge, MA, USA). The resulting plasmid pool was purified from transformed DH5alpha bacteria and transfected into two human neuroblastoma cell lines, SH-SY5Y and SK-N-BE(2). Each transfection was repeated six independent times. Both plasmid DNA and cellular RNA were extracted from the cells 42 h post-transfection. Plasmid DNA and cDNA sequences containing the cloned SNPs were amplified using PCR primers that also incorporated sample barcodes, unique molecular indices (UMI), and Illumina-sequencing adapters. The resulting PCR products were sequenced using one lane of Illumina HiSeq 4000 using a 75 bp paired-end
