DNA sequences were analyzed by SeqScape v2.6 (ABI). Automated sequence analysis was performed as follows to validate the results from SeqScape: Sequence chromatograms were loaded into CodonCode Aligner (CodonCode Corperation) to determine homozygous and heterozygous calls using the built-in phred program. Phred-derived sequences were then converted into fasta, and uploaded into the Galaxy (35) tool for sequence alignment against the CHRNA4 genomic sequence.
