It should be noted that the correlation between ethanol-induced conditioned taste aversion and ethanol intake in a two-bottle choice paradigm was shown for the initial (after first injection of ethanol) changes in saccharin consumption (Broadbent et al., 2002). Therefore, changes in saccharin intake for trial 1 compared with Trial 0 were calculated, and the results are presented in Fig. 8B. Although these data showed no main effect of pretreatment but only a main effect of dose [F(1, 46) = 55.4, p < .001] and pretreatment × dose interaction [F(1, 46) = 7.8, p < .01], post-hoc analyses showed significant differences between both ethanol-injected groups of mice (p < .05), indicating a smaller reduction in saccharin intake after pretreatment with LPS. Taken together, these results indicate that LPS pretreatment reduces ethanol CTA.
